Methodology
Immunoassay (IA); Photo-Optical Clot Detection
Specimen Type
Plasma
Performing Lab
Quest Diagnostics San Juan Capistrano
Epic ID
Other Reference Test
Collection Information
Specimen Required
(two-four) 1.8 mL or (two-three) 2.7 mL Blood
Container Type
Light Blue (Sodium Citrate)
Processing Information - Lab Use Only
Transport Temperature
Frozen-Only
Specimen Stability
Room Temp=Unacceptable; Refrigerated=Unacceptable; Frozen=30 Days
Rejection Criteria
Hemolysis; Grossly lipemic; Serum
Test Volume Required
3 mL Platelet-Poor Plasma
Minimum Test Volume
2 mL Platelet-Poor Plasma
Processing Instructions
Platelet-poor plasma: Centrifuge light blue-top tube 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/uL). Freeze immediately and send frozen.
Included Tests
Cardiolipin IgG,IgA,IgM; B2-Glycoprotein I IgG, IgM, IgA and Lupus Anticoagulant Evaluation with Reflex. (PTT-LA and dRVVT with Reflex Confirmations) If the PTT-LA test is prolonged (>40 seconds), then Hexagonal Phase Confirm is performed. If the Hexagonal Phase Confirm is positive or weakly positive, then a Thrombin Clotting Time will be performed. If dRVVT screen is prolonged (>45 seconds), then dRVVT Confirmation will be performed. If dRVVT Confirm is positive, then a dRVVT 1:1 Mix will be p
CPT Codes
86147 (x3), 86146 (x3), 85730, 85613; 85598, 85670,85597; 85613
Revised
02-26-20
Initials
SL